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Paraformaldehyde at a concentration of 4% v/v (pH 7.4) was used to fix the cells for 10 min in the dark after being washed with phosphate-buffered saline (PBS, pH 7.4) 3 times
Clean the spot first to stop infections
Finally, Patience is Key
Glutathione (BP)........................................1g
We provide multi-level evidence that CydDC is a heme transporter required for functional maturation of cytochrome bd , a pharmaceutically relevant drug target
Morphology (the optical density of the small colonies is considerably higher) is used as a supporting determinant
