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In Vitro Pull-Down Binding Assays To determine whether GSTP binds directly to HMGB1, recombinant polyhistidine-tagged GSTP (WT), GSTP (S184A), GSTP (S184D), and HMGB1 proteins were expressed in Escherichia coli BL21 (DE3) and purified through Ni-NTA spin column (Qiagen)

As expected, we observed that the loss-of-function mutant form of hHPN RS exhibited higher protein levels compared to the wild-type hHPN WT , both in serum and liver lysate

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3 integrin and Src facilitate transforming growth factor- mediated induction of epithelial-mesenchymal transition in mammary epithelial cells

-For 200 mg, break the score on either the left or right side of the tablet (two-thirds of a tablet)