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Journal of Investigative Dermatology 117(6):1350-1356. [doi.org]

10.1124/jpet.102.047928 240 SoykaM.MutschlerJ

From the density analysis, the only possible binding region of citrate is located at the peptide position from D604 to K617

Calcium influx was measured using flow cytometry for 5 min on a BD FACSymphony A5 flow cytometer, and calcium flux was quantified as the ratio of Indo-1 emission at 400 nm (bound Ca 2+ ) to 475 nm (free Ca 2+ )

And elevated NAD+ activates sirtuins (especially SIRT1 and SIRT3), the enzymes that shift cellular metabolism from carbohydrate dependence to fat oxidation