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At low micromolar concentrations, inhibition of ALDH2 by 4-HNE is reversible, but at higher concentrations, when the active site thiols are covalently modified, inhibition becomes irreversible (136)
Western Blot Analyses Total proteins from mouse liver tissues and cells were extracted with T-PER lysis buffer (#78510, Thermo Scientific, New York, NY, USA) and M-PER lysis buffer (#78505, Thermo Scientific, New York, NY, USA) supplemented with protease and phosphatase inhibitors (#C0001 and C0004, Targetmol, Boston, MA, USA)

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Haber AL, Biton M, Rogel N, et al

These antioxidant functions have many downstream effects, allowing EGCG to effectively inhibit the hazardous reactions that can occur if free radical concentration is dysregulated
