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There are three enzymes contributing to UPP: E1 (Ub-activating enzyme) and E2s (Ub-carrier) prepare Ub for conjugation, but the main enzyme in the process is the E3 (Ub-protein ligase) which transfers the activated ubiquitin to the protein substrate to be degraded [66]

Unlike the tetrameric GPx1-GPx3, the GPx4 protein is a monomer which can not only reduce hydrogen peroxide, but also distinctly react with lipid and fatty acid hydroperoxides including cholesterol derivatives, amongst many other substrates

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Rossjohn, J., McKinstry, W.J., Oakley, A.J., Verger, D., Flanagan, J., Chelvanayagam, G., Tan, K.L., Board, P.G., Parker, M.W

J Frailty Aging