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The LC-MS/MS analysis was performed using a Sciex QTRAP 6500 + - MS connected to a Sciex AD UHPLC

( e ) FT282-CCNE1 cells transduced with shRNA targeting CrAT, CrOT, or TMLHE (shTMLHE) were incubated with 13 C 6 -glucose, and glucose-derived M + 2 acetyl-CoA was assessed (n = 8 technical replicates representative from 2 independent experiments)

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