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After collection, the samples were centrifuged at 2000 g at 4 C for 10 min
In: Brehlin M., editor

Worms age synchronization Step 1 : Worms were chunked onto a pre-seeded 10 cm NGM plate and allowed to grow up to 23 days until large numbers of eggs and gravid adults were apparent on the plate

Nureki O Structural biology of the multidrug and toxic compound extrusion superfamily transporters
Biochim Biophys Acta 30(3):513521 Scott DL, White SP, Otwinowski Z, Yuan W, Gelb MH, Sigler PB (1990) Interfacial catalysis: the mechanism of phospholipase A2