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Pei Y, Liu H, Yang Y, Yang Y, Jiao Y, Tay FR, et al

doi: 10.1016/j.pupt.2016.03.003
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This chronic activation impairs neuronal homeostasis, worsens synaptic dysfunction, and ultimately accelerates neurodegenerative processes [113116]

This aligns with the reduced smooth muscle content and decompensation observed in the bladder walls of rats with pBOO, in which apoptosis was associated with mitochondrial dysfunction
Anti-Inflammatory and Cytoprotective Actions Inflammation is the bodys defense mechanism, but prolonged inflammation delays recovery and causes further tissue breakdown

Proteins were separated by SDS-PAGE and transferred onto a PVDF membrane (Immobilon-P, Millipore), blocked with 5% skim milk, and incubated with antibodies
