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In 1986, the respective roles of HSL and MGL in TAG hydrolysis were clarified when isolated HSL was found to hydrolyze acylglycerol with an accumulation of MAG in vitro, whereas glycerol release was blunted through selective removal of MGL by immunoprecipitation (31)

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Although sometimes sterile water can be used multiple times in the 24-hour window, depending on its use

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