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Materials and methods Cell culture and transient transfection HeLa, 293FT, DU145, and H1299 cells were cultured in Dulbeccos modified Eagles medium (DMEM, WelGENE) supplemented with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (Gibco)

To demonstrate the use of HisLink Resin on an automated platform we used an AKTA explorer from GE Healthcare to purify milligram quantities of polyhistidine-tagged protein from 1 liter of culture

H., Baird, D

Transplantation (2013) 95:9605

Imbalances in calcium levels can disrupt various physiological processes, including muscle contraction, nerve transmission, blood clotting, and bone health