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Indirect method The free (unencapsulated) LC was separated from the nanoparticle fraction by centrifugation of the nanosuspension at 9,500 rpm for 30 min at 4 C
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Additionally, a randomized controlled clinical trial in patients with stroke did not show any difference in LC supplementation between 1000 mg/d and 2000 mg/d [95]
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After incubation for 72 h, cell viability was measured and was decreased in a dose-dependent manner (Fig
