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This is in contrast to the postpubertal stage, where the reduced ER levels prevent the ER/IRS1 complex formation, and alter the signaling via the Raf/MAPK pathway [110]
Hematoxylin-eosin (HE) staining HE staining was performed as previously reported [25].The tumor specimens were cut to about 4 m sections and embedded in paraffin for hematoxylin-eosin staining
For pigmentation and skin rejuvenation, treatments are generally spaced 3-4 weeks apart
