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Total proteins were quantified by BCA Protein Assay Kit, separated on 10% SDS-PAGE, and then transferred onto polyvinylidene difluoride membranes (BioRad)

This work identifies peroxisomal fatty acid oxidation as a critical metabolic pathway in DTP cells, as a consequence of the metabolic switch towards oxidative phosphorylation that has been described in DTP cells from multiple tumour types

In vivo sepsis model Sepsis was induced in C57BL/6J mice by intraperitoneal (IP) injection with LPS (10 mg/kg) for 2 hr followed by 100 mM/100l, pH 7.4 ATP (Sigma-Aldrich, A7699) IP injection as previously described (53)

2001 Jun;4(2):143153