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Using a design of experiments (DOE) framework, we systematically evaluated combinations of extracellular matrix proteins and soluble factors to identify conditions that reduce myofibroblastic marker expression and extracellular matrix production
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These challenges include sampling (spot urine, 24 h urine, plasma, serum etc.) and analytical issues (missing reference standards, matrix effects, protein adducts, recovery, limit of detection, stability during storage, etc.)