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However, by employing additional steps such as blocking endogenous biotinylated proteins with free streptavidin, subcellular fractionation, or extra washes, this method may potentially be applied to study proteins in the cytoplasm or plasma membrane [115]

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Direct protection is offered by antioxidants: glutathione (GSH), ascorbate (vita-min C), tocopherol (vitamin E), and carotinoids

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Whats happening: Glutathione is supporting cellular repair across multiple systems