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The cells were seeded on a 24-well plate with an initial density of 50,000 cells/well and incubated at 37 C in 5% CO 2 overnight

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Given the role of GPx1 in promoting cell survival pathways, the effectiveness of other chemotherapeutic agents, such as the isothiocyanate iberin, may be due, in part, to their ability to decrease GPx1 expression
