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The mechanism involves time-dependent miR-21 reduction at 1, 3, and 24 h post-treatment, though PTEN protein expression remains unchanged despite miR-21 suppression, suggesting complex regulatory networks (64)

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When the TC1-6 cell line was transduced with adenovirus expressing sh-VGF, we observed that the glucagon granules formed larger and concentrated foci near the nuclear periphery, which were distinct from their distribution in the sh-Control group (Fig