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The participants consumed relatively large amounts of nicotine-containing e-liquid (almost half a bottle per day)

Proper usage, such as not swallowing excess saliva, helps prevent unnecessary irritation to the digestive lining

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This finding suggests that Sertoli cell dysfunction, as reflected by anti-Mllerian hormone production, may play a role in maintaining chromatin stability during spermiogenesis. Other hormones, including follicle-stimulating hormone, luteinising hormone, oestradiol, inhibin B, and thyroid-stimulating hormone, did not show associations with semen parameters or DNA fragmentation