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When a pregnancy is planned it is best to quit smoking in advance of conception but in the case of unplanned pregnancies it can be quite a shock and extremely worrying for the expectant mother who smokes

To account for the crossover design and repeated measures, linear mixed-effects models with fixed effects for period and exposure sequence (ie, filtered then unfiltered or unfiltered then filtered) and with random effects for sex and participant will be used to analyze continuous outcomes and changes from baseline