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HC, DS and MM contributed to the interpretation of the data, and revising the manuscript

They are most active from midsummer to late autumn

They play in the fields, and they help their mother work, particularly during the burley tobacco harvest when she is paid a piece rate of about 22 cents for each stick of tobacco plants she harvests and hangs in a barn

Many people tend to think of tobacco in terms of secondhand smoke, which is important to be sure, but it is not often that the impact of those cigarette butts, e-cigarette waste, snuff and snus pouches, and other tobacco residue is actually having on natural areas and wildlife when people use them in natural settings

Sample collection Samples were collected by the National Agency for Sanitary Surveillance (ANVISA) in Brazil and provided to the Centers for Disease Control and Prevention (CDC) for chemical analysis and characterization

E-cigarettes were classified as tobacco products in 22 countries