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James EA, Wang C, Wang Z, Reeves R, Shin JH, Magnuson NS, Lee JM (2000) Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells

Reverse transcription quantitative polymerase chain reaction RNA was extracted from post-caval lung tissue using TRIzol reagent (Fisher Scientific) according to manufacturer instructions

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[PubMed: 4003920] Tager IB, Segal MR, Speizer FE, Weiss ST

This results in increased goblet cell numbers associated with sputum production and increased epithelial thickness linked to breathlessness [42]