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3D ALI models are constructed using primary cells which differentiated over a period of time to form multi-cell populations and are more similar to human tissues, while 2D ALI models mostly use single cell type, especially cell lines

Proc Natl Acad Sci USA 111:51355140 Qin L, Mo N, Muhammad T, Liang Y (2018) Genome-wide analysis of DCL, AGO, and RDR gene families in pepper ( Capsicum annuum L.)

Smoking introduces nicotine and other harmful substances into the body, affecting multiple systems, including the endocrine system, which is responsible for hormone regulation

The health effects over multiple seasons are not yet clear

Passel and Jens Manuel Krogstad, What We Know about Unauthorized Immigrants Living in the U.S., Pew Research Center, November 16, 2023, [11] Anita Alves Pena, Legalization and Immigrants in U.S

Mapacho has been used since ancient times to connect the Four Directions, Eastern Air to breathe us life, Southern Fire for transformation, Northern Earth for stabilizing energy of the ground, and Western Water for reflection
