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Specific primers for each gene (Supplementary Table 3) were designed using Primer Express software (Applied Biosystems)

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and Advised to avoid or reduce the amount of time spent with people, places or social situations that might have been strongly associated with smoking

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Researchers pooled data from 141 studies done in 21 countries and regions, together involving millions of people

A Spanish study published in 2022 highlighted the growth in sales of IQOS heated tobacco sticks from the manufacturer Philip Morris, while a decrease in sales of conventional cigarettes was recorded in Spain.[3]This study suggested that tobacco manufacturers' messages that heated tobacco would be less harmful than conventional cigarettes should be better controlled