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Funnel plots performed for outcomes retrieved in at least 10 studies ruled out the possibility of publication bias (Figures S14, S16, S18, S20, S22, S24, S26)

Scheen has received lecturer/scientific advisor/clinical investigator fees from AstraZeneca, Boehringer Ingelheim, Eli Lilly, Merck Sharp & Dohme, and NovoNordisk, and has worked as a clinical investigator in the EMPA-REG OUTCOME, CANVAS-R, DECLARE-TIMI 58, LEADER and HARMONY Outcomes cardiovascular outcome trials

Preclinical evidence suggests that increasing protein intake may offset circulating amino-acids reduction, however this may also blunt the increase in whole-body energy expenditure [63]

Maximum Weight Loss Retatrutide Phase 2 data showed 24.2% mean weight loss at 48 weeks with 12 mg dose, the highest weight reduction reported for any single anti-obesity agent in Phase 2 trials, exceeding survodutide's 18.7%

qRT-PCR was then performed using iQ SYBR Green Mix (Bio-Rad, Hercules, CA)