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[DOI] [PubMed] [Google Scholar] 613.Baggio L.L., Yusta B., Mulvihill E.E., Cao X., Streutker C.J., Butany J

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The overexpression of PFKFB3 has been found not only in cultured lung fibroblasts stimulated by inflammatory cytokines [35, 45, 50], but also in myofibroblasts from IPF patients [12] and in lung tissues from different animal models of pulmonary fibrosis [12, 50]

Both approaches carry risks, but the risks are different

Subsequently, the supernatant was collected and centrifuged repeated at 10.000 g at 4 C for 10 min to improve purification from cell detritus and platelets