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The cDNA samples and primers were combined with SsoAdvanced Universal SYBR Green Supermix (BioRad) and RT-qPCR was conducted using the CFX384 Touch Real-Time PCR System (BioRad)

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While GLP-1s are currently permitted in sport, its important for athletes to know that the World Anti-Doping Agency (WADA) is monitoring the use of GLP-1 agonists by athletes to determine if they should be prohibited in the future

This helps regulate appetite and blood sugar levels (glucose), but it could also cause nausea

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