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cDNA was further amplified for 29 cycles with Q5 (NEB, M0491) and diluted 3 times for qPCR

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GIP receptor activation enhances insulin sensitivity and appears to reduce some of the gastrointestinal side effects associated with pure GLP-1 agonists

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These findings prompt speculation regarding the existence of a threshold for the reduction of GLP-1 necessary to promote locomotion, suggesting a finely tuned control mechanism within the gut