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They were transiently transfected with truncated GLP-2 receptors attached to LgBiT and G-SmBiT (or G-HiBiT) in a ratio of 1:1 using Lipofectamine 2000 transfection reagent (Invitrogen) and incubated for 24 h

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Using GLPLight1, they also explored the spatial information of GLP1R activation after uncaging photo-GLP1, finding that GLPLight1 could detect GLP-1 responses with high spatial resolution, and this response could be confined to specific regions of the cell membrane