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These next-generation constructs build upon the foundational architectures of earlier iterationsspecifically, the integration of a single costimulatory domain (e.g., CD28 or 4-1BB) in second-generation CARs and the inducible immunomodulatory capacity (e.g., cytokine secretion) of fourth-generation TRUCKs

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Limited material availability necessitated that the off-target analysis and 5-RACE assay be performed with one sample per group