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The membrane sample with GLP-1R:Gs:Nb35 complex formed was collected by centrifugation at 37,800g, and then solubilized by in a buffer comprised of 1% DDM, 0.5% CHAPS, 0.2% Cholesteryl hemisuccinate (CHS), 30 mM Hepes, pH 7.8, 150 mM NaCl, 30% glycerol, 25uM TCEP, 2.5 mg/ml Leupeptin, 0.16 mg/ml Benzamidine, and 1uM GLP-1

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[DOI] [PMC free article] [PubMed] [Google Scholar] 1250.Garg S, Kim L, Whitaker M, et al

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