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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

Before the initiation of the study, written informed consent was acquired from each of them, and the study was conducted in compliance with the Declaration of Helsinki, Good Clinical Practice guidelines, and national regulatory requirements

Scientific evidence of diets for weight loss: different macronutrient composition, intermittent fasting, and popular diets

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(5) Mice lacking the complement regulator gene, decay accelerating factor (DAF), are more susceptible to experimental myasthenia [67]