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Membranes were blocked in 5% (w/v) skim milk in tris-buffered saline with 0.1% Tween-20 for 1 h at RT, incubated overnight with a mouse monoclonal histidine tag antibody (MA1-21,315, Invitrogen) at 1:1000, and for 1 h at RT with a goat secondary anti-mouse IgG antibody conjugated with alkaline phosphatase (A3438-25ML, Sigma-Aldrich)

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