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We were able to refold exon 3 of UL37 from HCMV, a MHC class I-like viral mimic, using this method, but not with regular dialysis or dilution methods despite multiple trials

Natural compounds derived from cyanobacteria, such as Anabaena sp., Nodularia sp., Nostoc sp., and S

General protein purification buffer considerations Buffer components to limit or avoid entirely For GoldBios His-Tag Buffer Set and His-Tag Column Prep Protocol, we recommend using 50 mM Phosphate Buffer pH 8, 300 mM NaCl, and 10 mM Imidazole as the binding buffer, and this is a great baseline buffer to use if youre working with a new protein and unsure what is important to include in your purification buffers

Plasmid DNA was purified using the Thermo Scientific GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, Massachusetts, United States)

Altex 39, 221234234