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Increased free water in the substantia nigra in idiopathic REM sleep behaviour disorder

The suspended brain cubes were centrifuged at 100 g, 4 C for 20 sec to remove medium, and washed once with the medium
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In their landmark study, Ross and Subramaniam emphasize that understanding binding interactions requires a detailed analysis of both enthalpy (H) and entropy (S), rather than focusing solely on the free energy change (G in kJ/mol))

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