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Each sample was incubated three times in parallel, and an equal volume of pre-chilled acetonitrile was added at preset reaction time points, such as 0, 5, 10, 15, 30 and 60 min

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Zeruo et al., 2022)

The solution pH was measured with a Metrohm Aquatrode Plus combination electrode, filled with a saturated KCl/AgCl solution, and calibrated before each titration at the temperature in question using standard pH 4 and 7 buffers
And 5-Amino-1MQ addresses NNMT overexpression to reactivate fat cell metabolism
