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Protein expression Colonies of Escherichia coli BL21 (DE3) harboring expression clones encoding recombinant GSTs M4-4, P1-1 and S1-1 were grown overnight in LB medium with 100 g ml 1 ampicillin, in a shaking incubator at 200 rpm, 37 C

ECAR was used to evaluate glycolytic activity

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