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Disruption of NCOA4 383522 binding to (GST-GATE16) 2 by ATG4B was assessed by measuring BLI response using an orbital shake speed of 1,000 rpm at 30C for a 100-s association step, with a dissociation step in BLI buffer for 150 s

[DOI] [PMC free article] [PubMed] [Google Scholar] 33.Friedmann Angeli J.P., Conrad M

Enzyme-engineered nonporous copper(I) coordination polymer nanoplatform for cuproptosis-based synergistic cancer therapy

Over-lap PCR was used to generate six GSTP phosphorylation-mimicking mutants (42)

"" indicates downregulation or decreased activity."" indicates upregulation or increased activity

For example, it can be used to track the unfolding of globular proteins by observing changes in their retention time, which corresponds to changes in their Stokes radius (Corbett and Roche, 1983
