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The TATA box-binding protein (TBP) was used as a control gene, as previously described.20 Target gene expression was normalized to TBP and the target gene expression was analyzed by the 2 formula
Goddi A, Carmona A, Park SY, Dalgin G, Gonzalez Porras MA, Brey EM, et al

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Most research indicates that standard creatine supplementation provides consistent benefits across the menstrual cycle, with no evidence suggesting that varying intake timing or dosage significantly enhances the effects in relation to hormonal fluctuations

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however, approximately 5% are still present in adulthood, especially those located on the distal extremities