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As an alternative, an affinity ligand can be placed on or within a support and entrapped through cross-linking or changing the ligands ability to leave this material [80,109113] For instance, the protein BSA has entrapped through cross-linking this protein with glutaraldehyde [109]

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Next, we generated luciferase reporter plasmids by introducing the promoter region (2000 bp to +185 bp) of the human AMPK gene into the pGL3-luciferase reporter plasmid (Fig

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S pibvajcm vkem se hladina glutationu v tle postupn sniuje, co me vst ke zvenmu oxidanmu stresu a pedasnmu strnut