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Quantitative polymerase chain reaction (qPCR) was performed using SsoAdvanced universal SYBR green supermix (Bio Rad), 5 ng genomic DNA, 10 M primers (mMitoF1: 5 CTA GAA ACC CCG AAA CCA AA 3, mMitoR1: 5 CCA GCT ATC ACC AAG CTC GT 3, mB2MF1: 5ATG GGA AGC CGA ACA TAC TG 3 and mB2MR1: 5 CAG TCT CAG TGG GGG TGA AT 3 3,93 and RNAse-free water in a 25 L reaction volume on a CFX96 opus 96 Real-Time PCR system (Bio-Rad)

Glia also play an important role in regulating the synthesis and reuptake of glutamate and thereby influence synaptic function and morphology 16,17

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A recent meta-analysis study reviewed twelve randomized controlled trials (RCTs) (n = 791) and found that ALC supplementation significantly reduced depressive symptoms in MDD as monotherapy and augmentation compared to placebo, with effect sizes comparable to standard antidepressants and a superior safety profile

#21 At 5G/day with caps.....can be pricey

The SMN1 and SMN2 genes are shown according to two models in Figure 2