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After washed with PBS twice, cells were harvested by trypsinization and resuspended in PBS plus 5% FBS and analyzed by flow cytometry

[DOI] [PMC free article] [PubMed] [Google Scholar] 121.Neelov I., Popova E., Khamidova D., Tarasenko I

Microscopic examination found no changes in liver, spleen, lung, kidney, brain, thymus, or reproductive organs

GPx-1 overexpression, however, is protective against apoptosis only in circumstances where there is a disruption in normal redox balance favoring oxidation ( i.e ., under conditions of oxidative stress)

GSH brain concentrations are not commonly measured using 1 H-MRS, because relative to other 1 H-MRS-detectable neurochemicals (e.g., creatine (Cr), choline, N -acetylasparate), GSH concentrations are substantially lower

LI-COR Biosciences) at room temperature for another 2 h