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The chamber is designed to separate the luminal side from the basolateral side of the epithelium and, to assess permeability, molecules are placed in the luminal chamber, and the flux of these markers to the basolateral side is measured as an indicator of permeability of the epithelium [41]

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For athletes and sport-competing individuals, a separate concern applies: BPC-157 is prohibited under the World Anti-Doping Agency's S0 category of non-approved substances, which covers any pharmacological agent not approved for human therapeutic use by any regulatory body