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Hepatic antioxidant enzymes To determine liver superoxide dismutase activity, liver samples were homogenized with 0.1 mL of 65 mM phosphate buffer (pH 7.8), and then centrifuged at 10,000 g for 20 min

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Peer review Peer review information Nature Reviews Nephrology thanks Holger Eltzschig, Masaomi Nangaku, and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

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In the second step, the dipeptide -Glu-Cys is combined with the amino acid glycine by glutathione synthetase (GSS) to form the tripeptide GSH (Bottino et al., 2021, Lu, 2013, Wu et al., 2004)