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Behav Brain Res 301:5562 Xu T, Ren D, Sun X, Yang G (2012) Dual roles of sulforaphane in cancer treatment

Increases in the expression of various molecules that have been noted in reactive astrocytes across multiple contexts include GFAP, vimentin, nestin (probably labelling proliferating astrocytes), synemin plectin, -crystallin B chain, monoaminoxodase-B (MAO-B), heat shock factor binding protein 1, complement C3, lipocalin 2, C-X-C motif chemokine ligand 10, SerpinA3N, LCN2 and others, 20,95,96,97,98,99,100,101,102,103 but it important to note that no single molecular marker (including GFAP) is an absolute, required and sufficient, indicator of astrocyte reactivity, and no molecular markers have yet been identified that reliably distinguish amongst different reactive astrocyte phenotypes

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CHAC1 colocalization with mitochondria was determined with The Coloc 2 Fiji plug-in to determine the correlative overlap (Pearsons Correlation between red (CHAC1) and green (Mito-Grx1-roGFP2) signals as an indication of CHAC1 colocalization with mitochondria

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SFN, derived primarily from broccoli and with absolute bioavailability of around 80% [57], shows promise as a nutrigenomically active compound capable of increasing several endogenous antioxidant compounds via the transcription factor, Nrf2 [58]