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However, after worms are fixed, Nile Red reveals lipid droplets in the intestine, hypodermis, and in the germline

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Methods Enzymol 378:273286

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To generate ground-truth data for CNN training, the FIB-SEM datasets were downsampled to isotropic 12 12 12-nm voxels, where on a fraction of NE (~1/6), locations of nuclear pores were manually annotated, and each pore was estimated as a sphere (diameter of 7 pixels)