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There are multiple possible synthetic routes to obtain different cereblon ligand derivatives, and subsequent attachment to linkers for the construction of the final cereblon-based PROTACs

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A direct comparison of the molecular weight of recombinant NS1 from the NS1-5 product with that of NS1 alone and the NS1-NS2A fusion protein confirmed that NS1 existed as an NS1-NS2A fusion protein in the NS1-5 product, without cleavage at the NS1-NS2A junction (Fig